Thermo Fisher CellInsight NXT High Content Screening

High Throughput Cell Based Screening Cell insight with Orbitor stacker



Optical bench: Thermo Scientific high efficiency epi-fluorecent optical train

Stage: Automated with plate insert.

Camera: High-resolution Photometrics X1 CCD, 14-bit camera, with large field of view and 4.54micron/pixel resolution.

Barcode Reader: Laser-scanning barcode reader.

Objectives: Olympus 10x (0.3NA) and 20x (0.45NA), Additional 2.0x and 4.0x

Light Source: Four color solid- state LED light engine for use with Hoescht, FITC, TRITC, and Cy5 fluorophores.

Software: HCS Studio

CellInsight Instrument PC: Dell Precision T5600, 825W, Genuine Windows 7 Professional, 64-Bit OS, Four Core Xeon E5-2643, 3.3GHz Processor, 8GB, DDR3 RDIMM Memory

Dell 24" High Resolution Widescreen Monitor.

Highlights of the CellInsight NXT:

Transmitted Light - designed to work with or without fluorescent labeling, the new CellInsight NXT allows users to explore more cell biology without the restriction of fluorescent dyes
Affordable - automated high content imaging for all budgets
Easy to Use - quickly build and optimize image-based assays with included iDev* software
Fast Performance - scan and analyze a 96-well plate in under 4 minutes
Quality - high quality imaging and virtually maintenance-free operation
Compact - fits on a bench and less than 2 ft. on all sides
Capabilities of the CellInsight NXT:

Make individual cell, subcellular and multicellular measurements
Measure area, size, shape and texture using intensity and location of probes
Make multiple measurements per object, over time if desired
Measure subpopulations and reject undesired objects automatically
Easy to Use Applications:

Comprehensively pre-validated and user configurable image analysis without scripting or programming
Exploratory assay development tools and specific biology optimized tools
Define biological thresholds for outputs based on control populations
Ability to create multi-parameter “gates” from individual output measurements