Thermo Fisher CellInsight NXT High Content Screening


High Throughput Cell-Based Screening Cell insight with Orbitor stacker

Optical bench: Thermo Scientific high-efficiency epi-fluorescent optical train

Stage: Automated with plate insert.

Camera: High-resolution Photometrics X1 CCD, 14-bit camera, with a large field of view and 4.54 micron/pixel resolution.

Barcode Reader: Laser-scanning barcode reader.

Objectives: Olympus 10x (0.3NA) and 20x (0.45NA), Additional 2.0x and 4.0x

Light Source: Four-color solid-state LED light engine for use with Hoescht, FITC, TRITC, and Cy5 fluorophores.

Software: HCS Studio

CellInsight Instrument PC: Dell Precision T5600, 825W, Genuine Windows 7 Professional, 64-Bit OS, Four Core Xeon E5-2643, 3.3GHz Processor, 8GB, DDR3 RDIMM Memory

Dell 24″ High-Resolution Widescreen Monitor.

Highlights of the CellInsight NXT:

Transmitted Light – designed to work with or without fluorescent labeling, the new CellInsight NXT allows users to explore more cell biology without the restriction of fluorescent dyes
Affordable – automated high-content imaging for all budgets
Easy to Use – quickly build and optimize image-based assays with included iDev* software
Fast Performance – scan and analyze a 96-well plate in under 4 minutes
Quality – high-quality imaging and virtually maintenance-free operation
Compact – fits on a bench and less than 2 ft. on all sides
Capabilities of the CellInsight NXT:

Make individual cell, subcellular and multicellular measurements
Measure area, size, shape, and texture using the intensity and location of probes
Make multiple measurements per object, over time if desired
Measure subpopulations and reject undesired objects automatically
Easy-to-Use Applications:

Comprehensively pre-validated and user-configurable image analysis without scripting or programming
Exploratory assay development tools and specific biology-optimized tools
Define biological thresholds for outputs based on control populations
Ability to create multi-parameter “gates” from individual output measurements


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